Nonspecific Protein Adsorption at the Single Molecule Level Studied by Atomic Force Microscopy

Nonspecific Protein Adsorption at the Single Molecule Level Studied by Atomic Force Microscopy

Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy.

Liquid tapping atomic force microscopy was used to study the nonspecific adsorption of horse spleen ferritin at a bare gold surface at single molecule resolution. The majority of ferritin molecules adsorbed irreversible on gold surfaces in accordance with the random sequential adsorption (RSA) mechanism frequently used to describe irreversible adsorption processes. However, the time-resolved da...

Interferon α-2a interactions on Glass Vial Surfaces Measured by Atomic Force Microscopy

Atomic force microscopy was used to study adsorption and adhesion peculiarities of interferon α-2a on glass and mica surfaces. The specific protein adsorption behavior as a function of the pH value was illustrated on mica by single molecule imaging, while adhesion forces between interferon molecules and inner surfaces of borosilicate glass vials were measured directly under aqueous buffer condi...

Exploring mechanism of xanthate adsorption on chalcopyrite surface: An atomic force microscopy study

In this work, adsorption of the potassium amyl xanthate collector on the pure chalcopyrite surface was studied by applying atomic force microscopy (AFM). The adsorption experiments were carried out at different concentrations of the collector and at diverse pH values in the presence or absence of exterior ions. The changes occurring in the surface morphology of chalcopyrite due to the collector...

Entropic Elasticity of Single Polymer Chains of Poly(methacrylic acid) Measured by Atomic Force Microscopy

We have directly measured the entropic elasticity due to the uncoiling of individual polymer chains of poly(methacrylic acid) (PMAA) using the atomic force microscope (AFM). Covalent attachment of one chain end to a substrate and sufficiently low chain grafting densities were achieved by using a mixed monolayer technique that involved the co-chemisorption of (mono)thiol-functionalized PMAA and ...